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A rapid one-step diagnostic for the detection of Respiratory Syncytial  Virus



About RSV

 

Respiratory Syncytial Virus (RSV) is a negative-sense, enveloped single stranded RNA virus which belongs to the paramyxoviridae family. The virion is variable in shape and size (average diameter of between 120 and 300nm).  It is highly contagious and causes infection of the lungs and breathing passages. Infections can range from very mild illness to serious respiratory tract infections, including bronchiolitis and pneumonia, which usually occur in the very young, the very old and those with weakened immune systems.

 

Epidemiology of RSV

 

RSV has a substantial social impact, economically and in terms of human suffering.  The highest rates of RSV infection occur in infants 2-6 months old, and is the major cause of serious lower respiratory disease, in children within their first few years of life.

 

Each year, RSV is responsible for estimated 125,000 paediatric hospitalisations within the USA. RSV infections can be fatal in premature babies less than 6 months old and in infants with chronic lung, heart or immune deficiency diseases.  It is responsible for approximately 2,500 deaths per annum. Infections are global and typically epidemics occur during the winter months, October through to February. Two thirds of infants are infected with RSV during their first year and infection is usually universal by the age of two. The mean cost of a RSV hospital admission in the USA is approximately $27,101.

 

Although RSV is primarily a children’s virus, adults can be infected with RSV as well, the signs and symptoms of which mimic the common cold.

 

Signs and symptoms

 

Symptoms of RSV vary on the severity of the infection, and where the virus is located in the respiratory tract.  Initially RSV affects the upper respiratory tract, and symptoms may include:

 

  • low grade fever (102°F) fever
  • rhinorrhea (runny nose – clear discharge)
  • nasal congestion
  • cough
  • decreased appetite.

 

As the infection travels down to the lower respiratory tract, it can cause inflammation of the lungs and more severe symptoms such as:

 

  • coughing/wheezing
  • difficulty breathing
  • rapid breathing
  • circumoral and nail bed cyanosis.

 

Risk factors of RSV infection

 

Factors that increase the risk of RSV infection:

 

  • Birth within 6 months before onset of RSV season
  • Low birth weight (<2500g)
  • Young siblings
  • Day care attendance
  • Being in a multiple birth
  • Passive smoke exposure
  • Family history of asthma

 

There are also risk factors for developing severe RSV infections.  Listed below are common risk factors for developing severe RSV that may require hospitalisation:

 

  • Age younger than 3 months at time of infection
  • Prematurity
  • Chronic lung disease
  • Congenital heart disease
  • Weakened immune system
  • Lower socioeconomic status
  • Male sex.

 

How RSV is transmitted

 

RSV is highly contagious.  The virus is transmitted by physical contact with infectious secretions through hand contamination and self-inoculation of eyes, nose or mouth. The infection can also be spread through the air, by coughing and sneezing.  The virus is unstable in the environment, surviving between 4-7 hours on environmental surfaces, but it does not survive well at ≥ 37°C.

 

The spread of RSV is also almost unavoidable due the period of viral shedding.  Viral shedding may occur one to two days before symptoms become apparent, and can last as long as two weeks after symptom onset.  For children with defective cellular immunity, this period is significantly greater.  In most patients the incubation period is 4-6 days.

 

Duration of RSV once infected.

 

RSV infection usually lasts between 7 and 14 days.  Infants that are hospitalised with lower respiratory tract illness usually spend 5 to 7 days in hospital.  The virus can infect the same person several times during a lifetime, but re-infections are usually less severe. After each RSV infection the body forms some immunity, but it is not complete.

 

Diagnosis of RSV

 

For confirmation of RSV, testing should positively identify RSV and rule out bacterial infection. Nasopharyngeal secretions containing epithelial cells are necessary for positive diagnosis of severe RSV infections.

 

Viral isolation and subsequent culture from respiratory secretions are considered the gold standard of RSV diagnosis; confirmation can take several days consequently delaying treatment time for patients that may need immediate/aggressive treatment.  Rapid diagnostic tests are the preferred method since results are available within hours and most clinical laboratories use antigen detection assays to diagnose infection. Chest radiographs are sometimes used to determine the severity of the illness.

 

Treatment of RSV

 

No medications exist to cure RSV and treatment is aimed at ameliorating symptoms.  Patients with severe RSV may require specialised respiratory therapy including humidified oxygen and sometimes mechanical ventilation.

 

Ribavirin aerosol (anti-viral) has been administered to hospitalised children with severe pneumonia (caused by RSV), and some investigators have used a combination of immune globulin intravenous (IGIV) with high titers of neutralising RSV antibody (RSV-IGIV) and ribavirin to treat patients with compromised immune systems.

 

Causes of RSV

 

RSV bronchiolitis and pneumonia are two severe illnesses caused by RSV.  RSV bronchiolitis is associated with clinical signs and symptoms of small airway obstruction.  Airway obstruction in young infants can be very dangerous, as they have smaller peripheral airways and can become life-threatening if it is not manage appropriately.

 

During their first RSV infection, between 25% and 40% of infants and young children have signs of bronchiolitis or pneumonia. Of these around 2% require hospitalisation.

 

Cure & vaccinations For RSV

 

At present there is only one FDA approved medication available for halting the growth of RSV (ribavirin).  No treatment currently exists that can completely eradicate or cure RSV.  Numerous clinical studies are researching the possibility of a RSV vaccine.

 

Prevention of RSV in High Risk Patients

 

In instances where children are at high risk for developing RSV (e.g. when a baby has been born prematurely, and has not received all the natural substances to fight RSV and other viruses from the mother) medications such as palivizumab (Synagis) or RSV immune globulin (RespiGam) may be used to prevent infection.  A new medication, Numax Anti-RSV Mab, an antibody against RSV, similar to Synagis, but has been proposed as being 10 times more effective.

 

Benefits of rapid RSV diagnosis

 

The added risk of RSV transmission, posed by the long viral shedding period in RSV, can be reduced by early diagnosis and treatment of the infection. Other benefits of rapid RSV diagnosis include:

 

  • Administration of appropriate treatment
  • Reduce the risk of lower tract respiratory infections
  • Reduce risk of pneumonia and bronchiolitis
  • Decrease morbidity associated with RSV in high-risk young children
  • Reduce the spread to others
  • Reduce hospital stays
  • Reduction in cost of hospital care.

 

 

Useful links:

 

Centers for Diseases Control & Prevention

 

The RSV Information Centre

 

The RSVMD Website

 

The RSV Prevention Information Centre