Clearview® Malaria P.f.
and Clearview® Malaria Combo
Clearview Malaria P.f. is designed as a
simple, rapid, qualitative and cost effective method for testing,
in vitro , the presence of Plasmodium falciparum
(P.f.) malaria in blood. The test is an antigen-capture assay
detecting presence of a specific soluble protein, histidine rich
protein II (PfHRP-II), which is present in, and released from,
infected red blood cells. The assay is intended for use with venous
or capillary whole blood and does not require additional
instruments.
Clearview Malaria Combo is
designed as a simple, rapid, qualitative and cost effective method
for testing, in vitro, the presence of Plasmodium
falciparum (P.f), Plasmodium vivax (P.v.), Plasmodium malariae
(P.m) and Plasmodium ovale (P.o.) antigens in whole blood. The
assay is intended for use with venous or capillary whole blood and
does not require additional instruments.
For full features and benefits of Clearview
Malaria P.f. and Clearview
Malaria Combo please visit the features and benefits
section or for more information and facts about Malaria please see
About Malaria section. Scroll down
for instructions on how to use the
Clearview Malaria P.f.
and Clearview Malaria
Combo tests.
How to Use Clearview® Malaria
P.f. and Clearview® Malaria
Combo
Capillary blood collection
a) Select the finger for
puncture, usually the side of the third or fourth finger. Clean
with
antiseptic and allow to air dry.
b) Puncture the finger with
a sterile lancet. Blood will well to the surface. Repeat procedure
on
another finger if necessary.
c) Touch the pipette supplied to the blood drop and allow the
blood to fill up to the first (5 μL)
line.
Venous blood collection
Collect venous blood into an EDTA or Heparin tube. Whole blood
samples should be tested as soon as possible after collection. If
it is not possible to perform the test immediately, blood may
be stored for up to 3 days at 2 - 30°C (36-86°F).
Clearview Malaria® P.f.
ASSAY PROCEDURE
Step 1
Allow the cassette and blood sample to equilibrate to
ambient temperature prior to testing.
Step 2
Just prior to use, open the the foil bag, exposing the
cassette.
Step 3
Transfer blood to the test cassette by gently touching the
nozzle of the pipette to the SMALL well.
Step 4
Place 5 drops of the reaction buffer in to the LARGE well. Allow
the reaction to proceed for 15 minutes.
Step 5
Read the results and dispose of the
cassette.
INTERPRETATION OF RESULTS
(Please refer to the
illustration)
- POSITIVE: Two red coloured lines
appear. Both the test (T) and the control (C) lines are
observed, demonstrating that P.falciparum antigen is
present.
- NEGATIVE: One red line appears in the control
line region (C). The control line is present but not the
test line, demonstrating the test was performed correctly but no
P.falciparum antigen is present.
- INVALID: Control line fails to appear.
Either no lines are observed or a test line without a control line.
Improper test procedure was carried out or reagents have
deteriorated. Re-test.
Clearview Malaria®
Combo ASSAY
PROCEDURE
Step 1
Allow the cassette and blood sample to equilibrate to
ambient temperature prior to testing.
Step 2
Just prior to use, open the the foil bag, exposing the
cassette
Step 3
Transfer blood to the test cassette by gently
touching the nozzle of the pipette to the SMALL well.
Step 4
Place 5 drops of the reaction buffer in to the LARGE well. Allow
the reaction to proceed for 15 minutes
Step 5
Read the results and dispose of the
cassette
- P.f. Malaria POSITIVE: Two or Three red coloured
lines appear. The middle test (T) line and top control (C)
line, or all three lines are evident. All three lines may also show
in a mixed infection.
| |
 |
|
|
|
 |
| |
P.f. only infection |
|
|
|
P.f. or mixed infection |
- NON P.f. MALARIA POSITIVE: Two red coloured lines
appear. The bottom test (T) line and top control (C) line
are evident.
- NEGATIVE: The control (C) line is
present but not either test line, demonstrating the test was
performed correctly but no malarial antigens are
present.
- INVALID: Control line fails to appear.
Either no lines are observed or a test line without a control line.
Improper test procedure was carried out or reagents have
deteriorated. Re-test.
Click here to see the
Clearview Malaria P.f. Pack Insert
Click here to see
the Clearview Malaria Combo Pack Insert