About iFOB
Many diseases can cause
hidden blood in the faeces. This is also known as Faecal Occult
Blood (FOB), Human Occult Blood, or Human Haemoglobin. In the early
stages, gastrointestinal problems such as colon cancer, ulcers,
polyps, colitis, diverticulitis, and fissures may not show any
visible symptoms, only occult blood. Traditional guaiac based
methods lack sensitivity and
specificity, and also have diet restrictions prior to
testing.1,2
The Clearview
iFOB One Step Immunochemical Faecal Occult Blood Test
Device (Faeces) is a rapid test to qualitatively detect low levels
of human Faecal Occult Blood. The test uses a double antibody
sandwich assay to selectively detect Faecal Occult Blood at 50
ng/mL or higher, or 6 μg/g faeces. In addition, unlike guaiac
assays, the accuracy of the test is not affected by the diet of the
patients.
The Clearview
iFOB One Step Faecal Occult Blood Test Device (Faeces) is
a qualitative, lateral flow immunoassay for the detection of Human
Occult Blood in faeces. The membrane is pre-coated with
anti-haemoglobin antibody on the test line region of the test.
During testing, the specimen reacts with the particle coated with
anti-haemoglobin antibody. The mixture migrates upward on the
membrane chromatographically by capillary action to react with
anti-haemoglobin antibody on the membrane and generate a coloured
line. The presence of this coloured line in the test line region
indicates a positive result, while its absence indicates a negative
result. To serve as a procedural control, a coloured line will
always appear in the control line region, indicating that the
proper volume of specimen has been added and membrane wicking has
occurred.
References
- Simon JB. Occult Blood
Screening for Colorectal Carcinoma: A Critical Review,
Gastroenterology, 1985; 88: 820.
- Blebea J, Mcpherson RA.
False-Positive Guaiac Testing With Iodine, Arch Pathol Lab Med,
1985;109:437-40.